Coupled amplification and sequencing of genomic DNA.
نویسندگان
چکیده
منابع مشابه
Sequencing of genomic DNA by combined amplification and cycle sequencing reaction.
BACKGROUND Despite considerable advances, DNA sequencing has remained somewhat time-consuming and expensive, requiring three separate steps to generate sequencing products from a template: amplification of the target sequence; purification of the amplified product; and a sequencing reaction. Our aim was to develop a method to routinely combine PCR amplification and cycle sequencing into one sin...
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Peptide nucleic acid (PNA) microarrays have been used for the analysis of unlabelled DNA molecules by time-of-flight secondary mass spectrometry (ToF-SIMS). PNAs were synthesised with an automated system in multi-well plates followed by the spotting full-length molecules only onto derivatised silicon surfaces using a common split-pin contact printing instrument. After DNA hybridisation, phospha...
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AIMS To evaluate the technique of multiple displacement amplification (MDA) for whole genome amplification from small volume blood samples before sequencing in a clinical test to identify haemoglobin gene mutations. METHODS Phage phi29 DNA polymerase was used to perform MDA, starting with either 1 micro l of blood or 1 ng of previously isolated blood DNA from 23 patients. The amplified produc...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1991
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.88.7.2815